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. 2017 May 31;7:190. doi: 10.3389/fcimb.2017.00190

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Copyright © 2017 Vallejo Esquerra, Yang, Sanchez and Omsland.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of CbΔpckA. CbΔpckA was characterized to determine overall growth characteristics, metabolic deficiency and nutrient requirements. (A) The parental strain, CbΔpckA, CbΔpckA (pJB-CAT), and CbΔpckA (pJB-CAT::pckA) with pckA under the control of a constitutive promoter showed indistinguishable growth characteristics in ACCM-2, a medium containing both glycolytic and gluconeogenic substrates. (B) CbΔpckA and CbΔpckA (pJB-CAT) did not replicate on gluconeogenic substrates in plain D-ACM. Complementation of CbΔpckA with pckA restored replication. (C) The parental strain but not CbΔpckA was able to replicate in plain D-ACM. When D-ACM was supplemented with glucose, glucose 6-phosphate, pyruvate or phosphoenolpyruvate (1.5 mM), only glucose allowed chemical rescue of CbΔpckA. Data were collected from 3 independent experiments and are presented as mean ± SD.