Figure 5.

Two-photon calcium imaging during a sensory-based perceptual task. The Arduino based Go/No-Go behavioral task was performed simultaneously with two-photon calcium imaging. (A) Once trained (80% success), dendritic Ca²⁺ activity was imaged using two-photon microscopy through a chronically implanted window. In this example, Ca²⁺ transients (right) were recorded from the dendrite ROI (inset) on left. (B) Ca²⁺ activity throughout the behavioral task was reported for 40 trials for the dendrite shown in (A). (C) Top, Ca²⁺ transients above a threshold (>3× standard deviation of the noise) are reported as ticks. Colored ticks correspond to colored traces in (B). Bottom, summed histogram showing the number of Ca²⁺ transients occurring at different epochs throughout the trial. Stimulus was presented at 2 s (red bar).