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. 2017 May 31;8:625. doi: 10.3389/fimmu.2017.00625

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Copyright © 2017 Mordoh, Pampena, Aris, Blanco, Lombardo, von Euw, Mac Keon, Yépez Crow, Bravo, O’Connor, Orlando, Ramello, Levy and Barrio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Serum reactivity to MelC of PRE and POST1 samples of patients (pts) participating in CASVAC-0401 study, detected by ELISA (unpaired t-test, ***p < 0.0001). Optical density (O.D._450 nm) from each sample is shown as O.D._450nm = O.D. pt serum sample − O.D. healthy donor serum sample. A total of 1/10 serum dilution is shown (A). Serum reactivity to autologous tumor cells of patient #006 was detected by ELISA. A total of 1/10, 1/100, and 1/1,000 serum dilutions are shown (B). Immunofluorescence staining of autologous patient #006 tumor cells with PRE and POST 1 sera showing cell surface reactivity (original magnification: 400×). Antibodies were detected by FITC-rabbit anti IgA, G, and M (DAKO). PRE, POST1, POST2, and POST3 correspond to samples obtained at 0, 6, 12, and 24 months from protocol start. F-UP (follow-up) sample was obtained 24 months after ending protocol participation (C).