FIGURE 2.
Effect of 0.01–10 μM trans-pellitorine on Bodipy-C12 uptake as a measure of fatty acid uptake in fully differentiated 3T3-L1 cells. Cells were pre-treated with different concentrations of trans-pellitorine for 30 min. Treatment with 100 nM insulin for 30 min was used as a positive control and increased fatty acid uptake to 139 ± 11.7% (p < 0.001, not shown in the figure). Statistics: mean ± SEM, n = 6 with three technical replicates each. Significant differences (p < 0.05) were assessed by one-way ANOVA with Holm–Sidak post hoc test and are marked with distinct lower-case letters.