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. 2017 May 31;8:316. doi: 10.3389/fphar.2017.00316

Table 2.

Results of the ddPCR analysis of selected miRNAs in mature 3T3-L1 adipocytes (untreated or vehicle control/0.1% EtOH- treated cells) in comparison to undifferentiated control cells.

Control EtOH control
miR-let-7a 6.20 ± 0.45 6.58 ± 1.70
miR-let-7b 26.5 ± 6.70 27.2 ± 8.04
miR-let-7d 9.80 ± 1.87 11.1 ± 3.06
miR-103 19.8 ± 3.87 24.1 ± 5.10
miR-143 29.2 ± 1.13 27.6 ± 6.17

A fold change of 0.5 or 1.5 respectively, was used as cut-off criteria for regulation. Fold change ± SEM of selected microRNA expression on day 9 after induction of differentiation vs. undifferentiated control cells (=1) analyzed by means of digital droplet PCR. n = 3.