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. 2017 May 11;7(5):110. doi: 10.3390/nano7050110

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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In vitro cell transfection efficiency analysis. (a) Representative fluorescence microscopy micrographs show the uptake of siRNA in HepG2 cells after 30 min incubation with the superparamagnetic iron oxide nanoparticles (SPIO)-loaded cationic amylose complexed with FAM-labeled scramble siRNA (CA-SPIO/FAM-RNA), folate-conjugated CA-SPIO/FAM-RNA (FA-CA-SPIO/FAM-RNA), FA-CA-SPIO/FAM-RNA plus FA and untreated cells (control). Scale bar: 100 μm; and (b) flow cytometric data of cells incubated with FA-CA-SPIO/FAM-RNA, CA-SPIO/FAM-RNA, FA-CA-SPIO/ FAM- RNA plus FA and untreated cells (control). *: p < 0.05 compared with FA-CA-SPIO/FAM-RNA group.