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Primers used in this study, modified from Klindworth et al. (2013) to include Illumina adapter overhang nucleotide sequences (in bold) and an inosine (I) in the 3′end of 341f primer instead of N (i.e., A or T or C or G).
| Primer | Sequence 5′–3′ |
|---|---|
| 341fI |
TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG CCTACGGGIGGCWGCAG |
| 785r |
GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG GACTACHVGGGTATCTAATCC |
