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Pu-erh tea extract inhibited the expression of the RANKL-induced osteoclast-specific proteins. NFATc1, c-Src, c-Fos, cathepsin K, and β-tubulin levels were detected by western blotting, and the results were quantified using AlphaView software. Representative images are displayed. Values are shown as the mean ± SEM of three independent experiments. ΔΔΔ P < 0.001 versus the control; and ∗ P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus only RANKL-treated cells.
