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. 2017 May 30;10:267. doi: 10.1186/s13071-017-2198-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — ROS levels along the growth cycle of Leishmania lines. Promastigotes (1 × 10⁷) were collected at early logarithmic (EL), mid logarithmic (ML), early stationary (ES) and stationary (S) phases of growth (2, 4, 6 and 10 days, respectively) and basal ROS levels were measured by means of an H₂DCFDA assay. Data are the mean ± SD of three independent experiments. Significant differences versus the respective control line were determined using Student’s t-test (*P < 0.05)