. 2017 May 30;15:10. doi: 10.1186/s12953-017-0118-0

Table 1.

Proteins differentially abundant over time as a result of the microbial challenge. The p-values for both variables: time and litter are given for each spot, and a list of proteins identified by mass spectrometry, together with theoretical molecular weights, isoelectric points, accession number and Mascot score

Spot	Protein	P- value Litter	P-value Time	Accession	Theoretical MW (kDa)/ Pi	Mascot score
Proteins that showed decreased abundance over time in challenged birds
534	Cytochrome b-c1 complex subunit 1, mitochondrial Alpha-enolase	0.042	0.042	gi\|507543754 gi\|6048768	53.4/6.58 47.6/6.17	910 641
530	Heat shock cognate 71 kDa protein Heat shock 70 kDa protein	0.041	0.0082	gi\|45384370 gi\|55742654	71.0/5.47 69.9/5.53	1504 812
30	Protein disulfide-isomerase A3 precursor dihydropyrimidinase-related protein /Collapsin response mediator protein CRMP-62	0.041	0.0057	gi\|45383890 gi\|3122036	56.5/5.76 62.7/5.96	1272 486
523	Adseverin	0.041	0.0033	gi\|347360989	81.1/5.53	519
556	Collagen alpha-2(VI) chain precursor Collagen alpha-1(VI) chain precursor l-caldesmon	0.041	0.0067	gi\|45384382 gi\|49225581 gi\|212243	110.3/5.66 109.0/5.63 59.0/8.66	1038 490 287
513	Transitional endoplasmic reticulum ATPase	0.041	0.0040	gi\|113206112	90.0/5.14	261
11	Heat shock protein 70 Heat shock cognate 71 kDa protein	0.043	0.012	gi\|30962014 gi\|45384370	70.1/5.66 71.0/5.47	599 307
522	Collagen alpha-1(VI) chain precursor	0.039	0.0033	gi\|49225581	109.0/5.63	532
24	T-complex protein 1 subunit epsilon	0.046	0.0044	gi\|60302774	60.2/5.53	941
88	B-creatine kinase	0.041	0.037	gi\|211235	42.5/5.78	937
12	Annexin A6 NADPH--cytochrome P450 reductase	0.041	0.017	gi\|50982399 gi\|307775405	75.6/5.57 77.3/5.42	1336 528
33	Protein disulfide-isomerase A3 precursor Plastin-3	0.039	0.0033	gi\|453838905 gi\|7530180	56.5/5.76 71.3/5.51	2873 97
77	Aminoacylase-1-like isoform 1 Protein disulfide-isomerase A3 precursor	0.046	0.0057	gi\|363738588 gi\|45383890	46.3/5.98 56.5/5.76	442 424
29	Tubulin alpha ATP synthase subunit beta, mitochondrial Protein disulfide-isomerase A3 precursor	0.041	0.0066	gi\|223280 gi\|71897237 gi\|5383890	46.3/5.0 56.7/5.59 56.5/5.76	451 140 135
509	Heat shock protein 108 Endoplasmin precursor	0.041	0.0067	gi\|194220334 gi\|45383562	91.4/4.98 91.7/4.83	524 523
520	Adenosine deaminase	0.041	0.0044	gi\|57529377	41.1/5.82	1131
Proteins that showed increased abundance over time in challenged birds
133	Smooth muscle gamma actin Beta-actin Actin, alpha skeletal muscle B isoform 2	0.041	0.017	gi\|2967678 gi\|63018 gi\|50803534	42.3/5.31 42.1/5.29 42.4/5.16	310 262 247
220	Calcium-binding protein p22	0.041	0.0057	gi\|56118996	22.5/4.97	345
40	Histone-binding protein RBBP4	0.041	0.029	gi\|45382339	47.9/4.74	56
84	Tropomyosin alpha-1 chain	0.041	0.023	gi\|45382323	32.9/4.73	484
102	Desmin	0.041	0.0044	gi\|2959450	51.7/5.30	330
552	Desmin Smooth muscle gamma actin Actin, cytoplasmic type 5 ATP synthase subunit beta, mitochondrial precursor Vimentin Heat shock cognate 71 kDa protein	0.041	0.0033	gi\|2959450 gi\|2967678 gi\|56119084 gi\|71897237 gi\|114326309 gi\|45384370	51.7/5.30 42.3/5.31 42.2/5.3 56.7/5.59 53.2/5.09 71.0/5.47	1193 1002 937 218 133 132
502	Apolipoprotein A-I	0.043	0.029	gi\|211159	30.7/5.58	1020
550	Alpha-enolase Hypothetical protein CJMB04_24e12 26S protease regulatory subunit7 Beta-enolase	0.046	0.0044	gi\|46048768 gi\|53135040 gi\|57525333 gi\|46048765	47.6/6.11 23.8/6.79 49.0/5.72 47.6/7.28	872 555 331 137
546	Actin, aortic smooth muscle	0.041	0.00444	gi\|71895043	42.4/5.23	1063
73	Beta-actin Smooth muscle gamma actin	0.046	0.023	gi\|63018 gi\|2967678	42.1/5.29 42.3/5.31	263 256
Proteins that show variable, yet significant changes in abundance over time in challenged birds
134	PREDICTED:pyridoxal kinase	0.048	0.0066	gi\|27465053	32.8/4.65	2136
126	Alpha-tropomyosin 2	0.046	0.0066	363728772	35.2/5.93	244