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. 2017 May 30;12:30. doi: 10.1186/s13027-017-0139-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — TRAIL-stimulation decreased the mRNA-levels of TLR4. Cultures of PANC-1 (a), BxPC-3 (b) and COLO357 (c) were stimulated with 300 ng/ml TRAIL and 300 ng/ml TRAIL + 1 μg/ml LPS for 24 h. Non-stimulated cell cultures served as controls. TLR4 mRNA levels were measured by quantitative realtime-PCR. Data were normalized to mRNA expression of a housekeeping gene, GAPDH, and shown as fold change compared to untreated cells. Means and standard errors of the mean are shown. *) p < 0.05; **) p < 0.01