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. 2017 May 30;18:419. doi: 10.1186/s12864-017-3801-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — a Artemis coverage and stack view of the IFITM locus in DF1 cells following pull down of the IFITM locus using SureSelect probes and sequencing with PacBio. The figure shows an intact locus and successful mapping of the IFITM locus against the Gallus gallus sequence reference, despite two gaps observed within the B4GALNT4 and IFITM3 genes. b Artemis coverage and stack view of the IFITM locus in DF1 cells following pull down of the IFITM locus using SureSelect probes and sequencing with PacBio. These reads were instead mapped against the new PacBio contig 2 sequence reference. As for the mapping above, two gaps (one partial) are observed within the B4GALNT4 and IFITM3 genes, although more reads cross the gaps, allowing full coverage. c Artemis coverage and stack view of the IFITM locus in turkey breast tissue following pull down of the IFITM locus using SureSelect probes and sequencing with Illumina MiSeq. The graph shows successful mapping of MiSeq reads despite using chicken probes to pull down the locus in turkey tissue. The white bars represent actual gaps in the turkey reference as published on both Ensemble and NCBI and to which the probes will not eventually map as gaps are shown in the reference as “NNN”