Fig 4. Characterization of MRP3 membrane vesicles.
Membrane localization of MRP3 over-expressing cells stained using polyclonal antibody (inset). MRP3 vesicles were co-incubated with [3H]-E217G (10 μM) for 3, 5, or 10 min at 37 °C to evaluate time-dependent MRP3-mediated E217G transport (mean ± SD; n=1 in triplicate). Activity of MRP3 vesicles and mock vesicles was tested at 5 min (mean ± SD; n=1 in triplicate).