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. 2017 May 30;36:72. doi: 10.1186/s13046-017-0534-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — FGFR1 activation inhibits autophagy. a LC3-I/II western blot analysis in H1581 NSCLC cells cultured O/N in normal medium, serum-free medium, or serum-free medium plus FGF2 (25 ng/ml, 2 h). b Quantification of LC3-II levels in (a); mean ± SD, n = 3, ***p < 0.001. c LC3-I/II western blot analysis in H520 cells in conditions shown in (a). d Quantification of LC3-II levels in (c); mean ± SD, n = 3, ***p < 0.001. e Representative images of GFP-LC3 puncta in H1581/GFP-LC3 cells in conditions shown in (a). Scale bars represent 25 μm. f Representative images of GFP-LC3 puncta in H520/GFP-LC3 cells in conditions shown in (c). Scale bars represent 25 μm. g Quantitation of GFP-LC3 puncta in conditions shown in (e); mean ± SD, n = 3, ***p < 0.001. h Quantitation of GFP-LC3 puncta in conditions shown in (f); mean ± SD, n = 3, ***p < 0.001