Table 2.
BBD and the observed responses for the independent variables of extraction conditions.
| Run | Independent variables | Responses | |||||||
|---|---|---|---|---|---|---|---|---|---|
| X 1 | X 2 | X 3 | X 4 | Y 1 | Y 2 | Y 3 | Y 4 | Y 5 | |
| 1 | 50 (0) | 80 (1) | 20 (0) | 4 (1) | 35.88 | 45.24 | 79.24 | 77.54 | 69.14 |
| 2 | 50 (0) | 80 (1) | 10 (−1) | 3 (0) | 34.02 | 43.07 | 85.49 | 87.71 | 57.76 |
| 3 | 50 (0) | 80 (1) | 20 (0) | 2 (−1) | 38.58 | 47.4 | 67.66 | 77.81 | 68.52 |
| 4 | 50 (0) | 60 (1) | 20 (0) | 2 (−1) | 33.8 | 47.33 | 71.93 | 87.45 | 67.17 |
| 5 | 30 (−1) | 70 (0) | 20 (0) | 4 (1) | 35.22 | 43.53 | 80.34 | 98.43 | 67.54 |
| 6 | 70 (1) | 80 (1) | 20 (0) | 3 (0) | 35.75 | 47.05 | 82.62 | 98.17 | 76.91 |
| 7 | 50 (0) | 70 (0) | 20 (0) | 3 (0) | 39.14 | 47.08 | 87.93 | 94.12 | 75.58 |
| 8 | 70 (1) | 70 (0) | 20 (0) | 2 (−1) | 32.88 | 47.36 | 69.25 | 98.10 | 71.22 |
| 9 | 30 (−1) | 70 (0) | 30 (1) | 3 (0) | 37.96 | 46.01 | 72.72 | 93.77 | 66.06 |
| 10 | 50 (0) | 70 (0) | 30 (1) | 2 (−1) | 40.74 | 45.89 | 75.41 | 83.32 | 67.78 |
| 11 | 50 (0) | 60 (1) | 30 (1) | 3 (0) | 33.5 | 48.05 | 81.25 | 94.12 | 55.17 |
| 12 | 30 (−1) | 70 (0) | 20 (0) | 2 (−1) | 38.71 | 46.96 | 67.13 | 81.41 | 77.12 |
| 13 | 50 (0) | 70 (0) | 20 (0) | 3 (0) | 39.27 | 48.65 | 87.28 | 98.25 | 77.72 |
| 14 | 50 (0) | 80 (1) | 30 (1) | 3 (0) | 39.13 | 41.76 | 82.16 | 91.69 | 67.75 |
| 15 | 70 (1) | 60 (1) | 20 (0) | 3 (0) | 33.41 | 47.73 | 73.82 | 78.36 | 55.70 |
| 16 | 30 (−1) | 70 (0) | 10 (−1) | 3 (0) | 27.88 | 35.99 | 85.25 | 98.71 | 64.39 |
| 17 | 70 (1) | 70 (0) | 30 (1) | 3 (0) | 35.45 | 41.21 | 74.28 | 98.65 | 82.37 |
| 18 | 50 (0) | 70 (0) | 10 (−1) | 2 (−1) | 30.88 | 35.84 | 68.02 | 97.72 | 72.73 |
| 19 | 70 (1) | 70 (0) | 20 (0) | 4 (1) | 34.49 | 44.47 | 71.83 | 88.23 | 77.84 |
| 20 | 50 (0) | 70 (0) | 20 (0) | 3 (0) | 38.03 | 47.99 | 88.05 | 98.02 | 75.03 |
| 21 | 30 (−1) | 60 (1) | 20 (0) | 3 (0) | 34.57 | 48.23 | 78.41 | 98.69 | 65.58 |
| 22 | 50 (0) | 60 (1) | 10 (−1) | 3 (0) | 29.83 | 37.74 | 78.82 | 91.81 | 51.10 |
| 23 | 50 (0) | 60 (1) | 20 (0) | 4 (1) | 36.6 | 45.53 | 66.04 | 93.98 | 55.45 |
| 24 | 50 (0) | 70 (0) | 20 (0) | 3 (0) | 38.88 | 48.04 | 86.05 | 96.89 | 77.13 |
| 25 | 50 (0) | 70 (0) | 10 (−1) | 4 (1) | 30.24 | 40.41 | 83.20 | 94.92 | 57.53 |
| 26 | 50 (0) | 70 (0) | 30 (1) | 4 (1) | 40.35 | 36.95 | 67.15 | 97.40 | 79.90 |
| 27 | 30 (−1) | 80 (1) | 20 (0) | 3 (0) | 36.15 | 45.05 | 87.74 | 75.61 | 52.48 |
| 28 | 50 (0) | 70 (0) | 20 (0) | 3 (0) | 39.37 | 46.7 | 81.27 | 95.87 | 74.11 |
| 29 | 70 (1) | 70 (0) | 10 (−1) | 3 (0) | 29.27 | 41.22 | 79.06 | 97.58 | 59.85 |
X 1, ethanol concentration (%); X2, temperature (°C); X3, Sample-to-solvent ratio (w/v); X4, extraction time (h); Y1, yield of extraction (%); Y2, total polyphenol content (%); Y3, PTP-1B inhibition rate (the sample concentration is 5 μg/mL, %); Y4, α-glucosidase inhibition rate (the sample concentration is 50 μg/mL, %); Y5, ABTS inhibition rate (the sample concentration is 8 μg/mL, %).