Fig. 1.

HDM induced bronchial epithelial hyperpermeability and E-cadherin and β-catenin delocalization. a and b) 16HBE cells were exposed to HDM (200, 400, or 800 U/ml) for 24 h. TEER values and FITC-DX were measured immediately. c and d) 16HBE cells were treated with HDM (200, 400, 600, 800 or 1200 U/ml) for 24 h or stimulated with HDM (400 U/ml) for 1, 3, 6, 9, 12, 15 or 24 h. The expression of E-cadherin and β-catenin proteins was detected by Western blotting analysis in total lysates. e The location of E-cadherin and β-catenin was detected by immunofluorescence staining. Data are mean ± SD of four independent experiments. ^* P < 0.05 versus con group