Fig. 4.

Inhibition of RhoA/MLC signaling alleviated HDM-induced bronchial epithelial barrier dysfunction. a and b Cells pretreated for 6 h with inhibitors of RhoA/MLC signaling, Y-27632 2HCl (10, 100 μM) and GSK429286A (10, 100 μM), were exposed to HDM (400 U/ml) for 24 h. TEER values and FITC-DX were measured immediately. c MLC and p-MLC were detected. d The expression of E-cadherin and β-catenin was detected. Data are mean ± SD of three independent experiments. e The location of E-cadherin and β-catenin was detected by immunofluorescence staining. Data are mean ± SD of four independent experiments.^* P < 0.05 versus con group; ^# P < 0.05 versus HDM group