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. 2017 May 30;18:111. doi: 10.1186/s12931-017-0593-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — HDM and hrHsp90α increased the levels of RhoA activity and MLC phosphorylation. a and b 16HBE cells were treated with HDM (200, 400, 600, 800, or 1200 U/ml) for 24 h or stimulated with HDM (400 U/ml) for 3, 6, 12, 24, or 48 h. MLC and p-MLC were detected. c Cells were exposed to HDM (400 U/ml), hrHsp90α (10, 40 μg/ml) or hrHsp90β (10, 40 μg/ml) for 24 h. MLC and p-MLC were detected. d Cells exposed to HDM (400 U/ml) for 1, 3, 6, 12, 24 h. Then RhoA activity was measured by Rho G-LISA RhoA activation assay kit^TM biochem kit. e 16HBE cells pretreated with 1G6-D7 (30 μg/ml) for 2 h and then stimulated with HDM (400 U/ml), hrHsp90α (10 μg/ml) or hrHsp90β (10 μg/ml) for 6 h. RhoA activity was measured. Data are mean ± SD of three independent experiments.^* P < 0.05 versus con group; ^# P < 0.05 versus HDM group

Inline graphic — HDM and hrHsp90α increased the levels of RhoA activity and MLC phosphorylation. a and b 16HBE cells were treated with HDM (200, 400, 600, 800, or 1200 U/ml) for 24 h or stimulated with HDM (400 U/ml) for 3, 6, 12, 24, or 48 h. MLC and p-MLC were detected. c Cells were exposed to HDM (400 U/ml), hrHsp90α (10, 40 μg/ml) or hrHsp90β (10, 40 μg/ml) for 24 h. MLC and p-MLC were detected. d Cells exposed to HDM (400 U/ml) for 1, 3, 6, 12, 24 h. Then RhoA activity was measured by Rho G-LISA RhoA activation assay kit^TM biochem kit. e 16HBE cells pretreated with 1G6-D7 (30 μg/ml) for 2 h and then stimulated with HDM (400 U/ml), hrHsp90α (10 μg/ml) or hrHsp90β (10 μg/ml) for 6 h. RhoA activity was measured. Data are mean ± SD of three independent experiments.^* P < 0.05 versus con group; ^# P < 0.05 versus HDM group