Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 1;130(9):1596–1611. doi: 10.1242/jcs.195412

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 5. — Effects of NEDD4 or MARCH8 silencing on BST2 subcellular localization. siRNA-transfected HeLa cells were permeabilized before fixation and immunostained with antibodies against BST2 (green) and transferrin receptor (TfR, early/recycling endosomes; red) (A) or HRS (endosomes/MVBs; red) (C) along with DAPI (blue) to visualize the nucleus of the cells. Cells were observed with a confocal microscope. Scale bars: 10 µm. (B,D) Colocalization between BST2 and TfR or BST2 and HRS was assessed by calculating the Pearson's correlation coefficient on eight images per condition. Each dot represents the Pearson's correlation coefficient of one image featuring at least three cells. Error bars represent the mean±s.e.m. from each image (n=3), ***P<0.001 (Student's t-test).