Table 2.
Enrichment (GSEA) of top Hallmark Gene Sets for genes over- or underexpressed in the Scale-biter relative to all other taxa. Genes were pre-ranked by log2 fold change prior to analysis
| 48 Hours Post Fertilization | |||||||
| Overexpressed in Scale-biter: | Underexpressed in Scale-biter: | ||||||
| Name | NES | Pvalue | FDR | Name | NES | Pvalue | FDR |
| Epith. Mesench. Trans. | 2.80 | 0.00 | 0.00 | E2F Targets | −3.60 | 0.00 | 0.00 |
| Hypoxia | 2.27 | 2.0E-03 | 0.01 | Myc Targets V1 | −3.23 | 0.00 | 0.00 |
| Kras Signaling Dn | 2.08 | 2.1E-03 | 0.02 | Dna Repair | −3.06 | 0.00 | 0.00 |
| Estrogen Resp. Early | 1.87 | 0.01 | 0.07 | G2M Checkpoint | −2.94 | 0.00 | 2.4E-04 |
| Coagulation | 1.86 | 0.01 | 0.06 | Oxidative Phosph. | −2.60 | 0.00 | 8.8E-04 |
| Tnfa Sig. via Nfkb | 1.81 | 0.02 | 0.07 | Myc Targets V2 | −2.53 | 0.00 | 1.1E-03 |
| Angiogenesis | 1.64 | 0.04 | 0.14 | Mitotic Spindle | -2.29 | 1.9E-03 | 3.6E-03 |
| Myogenesis | 1.64 | 0.03 | 0.12 | Interferon Alpha Resp. | −1.83 | 0.01 | 0.04 |
| P53 Pathway | 1.58 | 0.06 | 0.14 | Mtorc1 Signaling | −1.70 | 0.03 | 0.07 |
| Kras Signaling Up | 1.54 | 0.05 | 0.15 | Fatty Acid Metabolism | −1.65 | 0.03 | 0.08 |
| 96 Hours Post Fertilization | |||||||
| Overexpressed in Scale-biter: | Underexpressed in Scale-biter: | ||||||
| Name | NES | Pvalue | FDR | Name | NES | Pvalue | FDR |
| Myc Targets V1 | 2.69 | 0.00 | 1.2E-03 | Bile Acid Metabolism | −2.59 | 0.00 | 2.0E-03 |
| G2M Checkpoint | 1.99 | 2.0E-03 | 0.07 | Myogenesis | −2.44 | 0.00 | 3.8E-03 |
| Androgen Response | 1.69 | 0.03 | 0.21 | Interferon Gamma Resp. | −2.01 | 0.01 | 0.05 |
| E2F Targets | 1.69 | 0.03 | 0.16 | Interferon Alpha Resp. | −1.75 | 0.02 | 0.15 |
| Myc Targets V2 | 1.68 | 0.03 | 0.13 | Inflammatory Response | −1.68 | 0.03 | 0.18 |
| Unfolded Protein Resp. | 1.47 | 0.07 | 0.29 | Adipogenesis | −1.66 | 0.03 | 0.16 |
| Notch Signaling | 1.44 | 0.10 | 0.28 | Protein Secretion | −1.54 | 0.06 | 0.24 |
| Heme Metabolism | 1.40 | 0.11 | 0.29 | Xenobiotic Metabolism | −1.51 | 0.07 | 0.24 |
| Mtorc1 Signaling | 1.25 | 0.18 | 0.47 | Oxidative Phosphorylation | −1.43 | 0.10 | 0.30 |
| Tnfa Signaling Via Nfkb | 1.11 | 0.32 | 0.69 | Kras Signaling Up | −1.41 | 0.10 | 0.28 |
| 8 Days Post Fertilization | |||||||
| Overexpressed in Scale-biter: | Underexpressed in Scale-biter: | ||||||
| Name | NES | Pvalue | FDR | Name | Nes | Pvalue | FDR |
| Uv Response Dn | 2.25 | 0.00 | 0.02 | E2F Targets | −5.90 | 0.00 | 0.00 |
| Il2 Stat5 Signaling | 2.14 | 0.01 | 0.02 | G2M Checkpoint | −4.88 | 0.00 | 0.00 |
| Kras Signaling Dn | 2.12 | 0.00 | 0.01 | Myc Targets V1 | −4.18 | 0.00 | 0.00 |
| Tnfa Signaling Via Nfkb | 1.80 | 0.02 | 0.07 | Oxidative Phosph. | −3.54 | 0.00 | 0.00 |
| Angiogenesis | 1.59 | 0.05 | 0.17 | Mtorc1 Signaling | −3.42 | 0.00 | 0.00 |
| Estrogen Response Early | 1.58 | 0.04 | 0.15 | Mitotic Spindle | −2.59 | 0.00 | 1.7E-04 |
| Notch Signaling | 1.54 | 0.06 | 0.16 | Fatty Acid Metabolism | −2.37 | 0.00 | 2.3E-03 |
| P53 Pathway | 1.45 | 0.09 | 0.21 | Dna Repair | −2.22 | 0.00 | 0.01 |
| Apical Junction | 1.45 | 0.09 | 0.18 | Interferon Alpha Resp. | −1.82 | 0.02 | 0.05 |
| Inflammatory Response | 1.42 | 0.10 | 0.19 | Glycolysis | −1.79 | 0.02 | 0.06 |
| 15 Days Post Fertilization | |||||||
| Overexpressed in Scale-biter: | Underexpressed in Scale-biter: | ||||||
| Name | NES | Pvalue | FDR | Name | NES | Pvalue | FDR |
| Inflammatory Response | 2.25 | 2.0E-03 | 0.02 | Oxidative Phosph. | −5.63 | 0.00 | 0.00 |
| Cholesterol Homeostasis | 2.13 | 2.1E-03 | 0.02 | Myc Targets V1 | −3.70 | 0.00 | 0.00 |
| Wnt B-Catenin Signaling | 2.02 | 3.9E-03 | 0.03 | Fatty Acid Metabolism | −2.52 | 0.00 | 0.00 |
| Hedgehog Signaling | 1.94 | 0.01 | 0.04 | Adipogenesis | −2.50 | 0.00 | 2.3E-04 |
| Pi3K Akt Mtor Signaling | 1.94 | 0.01 | 0.03 | Dna Repair | −2.46 | 0.00 | 1.9E-04 |
| Allograft Rejection | 1.83 | 0.02 | 0.05 | E2F Targets | −2.32 | 0.00 | 2.6E-03 |
| Uv Response Dn | 1.72 | 0.02 | 0.08 | Bile Acid Metabolism | -2.19 | 1.9E-03 | 0.01 |
| Complement | 1.70 | 0.03 | 0.07 | Tnfa Signaling Via Nfkb | −1.71 | 0.02 | 0.09 |
| Estrogen Response Early | 1.50 | 0.08 | 0.16 | Xenobiotic Metabolism | −1.55 | 0.06 | 0.17 |
| Apical Junction | 1.41 | 0.10 | 0.23 | Spermatogenesis | −1.51 | 0.07 | 0.19 |
Shown are the top 10 genesets shown for each analysis. Gene sets significant at FDR ≤ 0.25 shown in bold
NES Normalized Enrichment, Pvalue Nominal P value, FDR False Discover Rate (Q value)