Fig. 3.

Supplementation of 50% L-WRN CM with crypt niche factors. Cat enteroids were passed to multiple wells at P12 and cultured with 50% L-WRN CM or with additional crypt niche factor(s) shown to modulate organoid growth in various platforms. The individual cultures were passed every 3-4 days and monitored for growth. Cultures were terminated when the number and growth of the enteroids dissipated (N/A). None of the crypt niche factors rescued the cat enteroids much beyond those grown with 50% L-WRN CM.