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. 2017 Apr 3;6(5):602–618. doi: 10.1242/bio.021758

Fig. 12.

Fig. 12.

PGCLC formation by mouse embryonic DRG cells. E12 mouse DRG explants were cultured for 2 or 8 days. Immunostaining was performed using anti-Blimp1 and anti-Oct4 on culture day 2 or 8. (A) Bright-field image of a culture treated with LIF/BMP2/FGF2 during the first 6 days and subsequently exposed to 10% Nu-serum and LIF/BMP4 for 2 days. (B) Anti-Blimp1-positive cells in the same field as A. (C) Anti-Oct4-positive cells in the same field as A. (D) DAPI nuclear staining of the same field as A. (E) Merged image of B-D. White arrowheads in B-E indicate cells expressing both Blimp1 and Oct4. Scale bar: 50 µm. (F) Culture schedules of E12 mouse DRG explants for inducing the formation of PGCLCs. (G) Percentage of cells expressing both Blimp1 and Oct4 per DRG cell colony. *P<0.05 (Student's t-test) compared to the cultures treated with LIF/ BMP4 for 2 days. Data are expressed as mean±s.e.m. of separate counts of 6-11 colonies (the number in parentheses above each bar).