Fig. 3.

Early endosome dynamics is impaired in CAPNS1-depleted cells. (A) Control and shCAPNS1 U2OS cells were grown on Petri dishes, treated with commercial baculovirus reagent expressing GFP-Rab5 protein and RFP-KDEL. 24 h later, the cells were analysed using a confocal microscope to detect the dynamics of Rab5-positive vesicles. Images were acquired every 2 min over a period of 50 min. The first (T0) and last (T50) pictures of each-time lapse experiment are shown. Scale bars: 20 μm. (B) Corrected total cell fluorescence (CTCF) levels of GFP-Rab5 measured on 100 fixed control and shCAPNS1 cells. The graph indicates the percentage of cells that show a CTFC ≥3×10⁶; the error bars represent s.d. of three different independent experiments; P<0.01.