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. 2017 May 31;11:318. doi: 10.3389/fnins.2017.00318

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Copyright © 2017 Echevarria, Formichella and Sappington.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-6 deficiency affects microglial coverage regardless of IOP. (A,B) Representative 60X confocal images depicting quantification of percent coverage of GFAP+ astrocytes (green; A, left) and Iba-1+ microglia (green; B, left). The area containing above background signal for GFAP and Iba-1 was highlighted in red (A,B; right) and divided against the total area of the image to obtain the percent coverage measurements. (C,D) Representative 60X confocal images depicting GFAP+ astrocyte (green; C) and Iba-1+ microglia (green; D) labeling in whole mount retina of microbead- and saline- injected WT and IL-6-/- eyes. (E) Boxplot of percent coverage of GFAP+ astrocytes in saline (white) and microbead (gray) injected WT (left) and IL-6-/- (right) eyes. No genotype or IOP dependent changes were calculated. (F) Boxplot of percent coverage of Iba-1+ microglia in saline- (white) and microbead- (gray) injected WT (left) and IL-6-/- (right) eyes. While an IL-6 dependent increase in percent coverage of microglia is seen in both saline and microbead- injected eyes, only an IOP dependent decrease is seen in microbead- injected WT eyes. (G) Boxplot of Iba-1+ microglia cell density (counts/mm²) in saline- (white) and microbead- (gray) injected WT (left) and IL-6-/- (right) eyes. IOP dependent decreases in microglia counts are evident in microbead- injected eyes are evident in both WT and IL-6-/- eyes. ^*p < 0.05. n = 6–8 images/eye/condition/genotype. Scale bars= 30 μm for all images. Dashed lines in boxplot indicate median value of data set.