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. 2017 May 30;23:30. doi: 10.1186/s40409-017-0120-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of ergosterol in the ROS production of trypomastigotes. Fluorescence of H₂DCF-DA was measured after 60 min of incubation. Fluorescence was quantified by calculating the mean percentage of untreated (0%) and sodium azide-treated (100%) trypomastigotes. ***(p < 0.001). A representative assay is shown