Table 1.

Antitrypanosomal activity of ergosterol and cytotoxicity against mammalian cells

Compound	IC50 (μg/mL) 95% CI trypomastigote	IC50 (μg/mL) 95% CI amastigote	CC50 (μg/mL) 95% CI macrophages	Selectivity index	Hemolytic activity (SD)
Ergosterol	51.3 (46.0–57.0)	>100	> 200	> 3.9	7.03% (±11.6)
Benznidazole	4.4 (3.7–5.1)	1.9 (1.2–2.3)	> 200	> 45.0	nd

IC₅₀: 50% effective concentration; CC₅₀: 50% cytotoxic concentration; 95% CI: 95% confidence interval; SI: selectivity index calculated as (SI = CC₅₀ mammalian cell/IC₅₀ trypomastigotes); nd: not determined