Figure 5.

Identification and confirmation of novel proteins expressed highly in vivo that localize to cysts. Differentially expressed genes from the in vivo foci and in vitro culture datasets were partitioned into nine gene clusters (A-I) based on JS distance (Materials and Methods). Many of the 475 differentially expressed genes classified using both Cuffdiff and Sleuth (Materials and Methods) are highly represented in the clusters from this study (A). Other host-induced or encystation-specific transcriptome studies (Palm et al., 2005; Roxstrom-Lindquist et al., 2005; Morf et al., 2010) have fewer genes represented in the clusters than those identified by partitioning analysis in the foci (B). Many genes in clusters A, B, and C are associated with encystation or oxidative stress (C). In (D), C-terminal GFP fusions of genes from cluster B and C also are associated with the cyst wall or the interior of the cyst as visualized using colocalization immunofluorescent images with the cyst wall protein 1 (CWP-1) antibody.