Figure 6.

Differentially expressed genes in in vivo foci are similar to mid-late encystation time points in in vitro encystation transcriptomes. In vitro and in vivo differentially expressed genes from this study are compared to the in vitro encystation transcriptome of Einarsson et al. (2016). (A) Compares the 475 differentially expressed genes in this study (red, upregulated in foci; blue, downregulated in foci) to the in vitro culture transcriptome (TYDK) and the transcriptomes of four time points of an in vitro encystation transcriptome study. Percentages indicate the number of similarly differentially expressed genes in the in vivo foci relative to that in vitro encystation time point. (B) Summarizes processes upregulated in in vitro culture, which are primarily trophozoite growth and division (above) as contrasted with cellular processes upregulated in the in vivo foci (green) that are more similar to those genes upregulated during in mid-to-late in vitro encystation.