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. 2017 May 31;8:915. doi: 10.3389/fpls.2017.00915

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Copyright © 2017 Liao, Peng and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Leaf image processing by LeafletAnalyzer. (A) A high resolution image of seven sequentially-ordered leaves, L1 to L6, and the juvenile leaf (JL) of 6-week-old Medicago truncatula plants, obtained using a flatbed scanner. Note: the terminal leaflet of L1 was dissected apart to avoid overlapping with lateral leaflets. (B) Trichome removal. The original image of a leaf (center) was changed to black and white images, showing before (left panels) and after (right panels) trichome removal. Insets show close-up views of leaflet margins highlighted by rectangles in red. (C) Separation of terminal, and left and right lateral leaflets by the software.