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Growth of T7 phage Δ4107. A. Host strain was BL21(λDE3), 0.1 ml of phage from a 10-8 dilution was plated. B. Host strain was BL21(λDE3) freshly transformed with pJW25. 0.1 ml of phage from a 10-7 dilution was plated. C. Host strain was BL21(λDE3) carrying pJW25 after 10 subculturings. 0.1 ml of phage from a 10-6 dilution was plated.
