Figure 4.

Effects of heparanase inhibitors on the kinetics of HSkMEC pseudovessels formation and junctions between them. Cells were incubated with heparanase inhibitors (200 µg/mL) on Matrigel either in the presence (black columns) or absence (white columns) of MCF-7 heparanase-rich supernatant. Angiogenesis kinetic was assessed by: the determination of pseudo-vessels formed between 0 and 7 h (a); and junctions formed between 1.5 h to 7 h (b) with photos taken every 30 min. Results are presented as the slope of a linear regression realized with number of pseudo vessels and junctions determined at each time with the Image J software (see Supplementary Materials). (c) The number of pseudo vessels (±SD) formed at t = 9.5 h. Inhibition of the angiogenesis development is specified for each compound tested and indicated as a percentage missing compared to the blank values. Complete kinetics from 0 to 19 h are presented in Supplementary Materials.