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. 2017 Apr 18;13(6):2839–2847. doi: 10.3892/etm.2017.4358

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Cell integrity, cell cycle and quantified sub-G1 phase of alcohol-injured BNL murine embryonic liver cells treated with PA2. Cells were protected dose-dependently by PA2. All samples were pre-treated with 10% alcohol for 1 h, except for the (A) negative control sample. The alcohol-containing medium was then replaced with fresh medium with either (B) 0, (C) 1, (D) 5, (E) 10, (F) 25 or (G) 50 µg/ml PA2 for an additional 24-h culture. Cell integrity (dot plot; x-axis, cell-size; y-axis, granularity) and accumulated DNA content (histogram plot) of cell cycle were obtained by flow cytometry. PA2 dose-dependently reduced the proportion of sub-G1 phase cells (quantitative data value) and normalized cell-integrity. The sub-G1 values are presented as mean ± standard deviation, n=3. *P<0.05 vs. negative control group; ^#P<0.05 vs. alcohol-containing medium group; PA2, procyanidin A2.