. 2017 Apr 18;13(6):2839–2847. doi: 10.3892/etm.2017.4358

Table I.

Serum biochemical analyses performed at 1, 3 and 6-week sampling periods in CCl4-intoxicated mice.

Serum component	Referenced range	House keeping control	Sampling week	Vehicle control	Silymarin	Low LPE	Middle LPE	High LPE
GOT (IU/l)	69–191	141.23±20.5	1	69.83±8.35^a,b	80.62±7.29^a,b	48.36±5.63^b	77.45±14.5^a,b	87.46±16.73^a
			3	4298.09±546.27^a	3982.85±504.79^a	1620.58±578.42^b	962.56±512.04^b	1879.40±585.41^b
			6	2099.56±361.43^a	2169.08±358.68^a	821.94±297.18^b	402.82±56.31^b	1168.6±389.25^b
GPT (IU/l)	26–120	28.09±4.19	1	43.83±9.67^b	39.85±2.88^b	33.09±4.79^b	46.09±3.96^b	86.92±19.22^a
			3	1455.51±402.45^a	1247.49±383.78^a,b	586.89±336.78^a,b	312.25±291.82^b	303.90±185.50^b
			6	2569.26±492.33^a,b	3214.47±527.85^a	1514.42±484.47^b,c	928.39±232.72^c	1620.00±433.67^b,c
T-chol (mg/dl)	63–174	55.86±4.72	1	124.58±11.05	114.62±5.40	102.55±9.77	101.27±9.86	106.38±8.39
			3	78.09±5.78^a,b	83.19±9.81^b	88.55±9.69^a	71.42±6.73^a,b	93.77±11.25^a,b
			6	112.28±9.11	102.14±8.02	116.67±9.44	111.42±8.26	92.93±8.88
TG (mg/dl)	26–145	23.86±4.82	1	26.33±2.91^c	28.62±2.68^b,c	32.27±2.57^b,c	54.36±5.66^a	38.85±4.23^b
			3	17.16±1.91^a,b	19.20±2.33^b	32.27±3.63^a	22.33±1.88^a	21.15±3.94^a
			6	32.26±7.09	27.64±4.00	45.61±11.50	37.85±4.32	27.33±6.99
T-p (g/ml)	4.3–6.4	4.07±0.42	1	4.93±0.31^b	5.88±0.26^a	4.31±0.26^b	4.83±0.22^b	4.90±0.27^b
			3	3.83±0.29^c	3.97±0.37^b,c	5.19±0.4^a	5.02±0.45^a,b	5.90±0.45^a
			6	5.56±0.42^a,b	5.32±0.28^a,b	5.51±0.30^a,b	6.20±0.32^a	4.64±0.22^b
Albumin (g/dl)	2.5–4.8	1.95±0.19	1	2.73±0.16^a,b	2.99±0.11^a	2.45±0.15^b	2.73±0.17^a,b	2.75±0.18^a,b
			3	2.04±0.18^b	2.14±0.19^b	2.84±0.24^a	2.69±0.26^a,b	3.01±0.26^a
			6	2.83±0.20^b,c	2.68±0.15^b,c	3.07±0.16^a,b	3.34±0.18^a	2.52±0.10^c
Globulin (g/dl)	0.6	2.12±0.24	1	2.23±0.18^b	2.89±0.19^a	1.85±0.15^b	2.17±0.17^b	2.15±0.17^b
			3	1.79±0.12^b	1.82±0.19^b	2.36±0.18^a,b	2.49±0.26^a	2.89±0.26^a
			6	2.73±0.23^a	2.64±0.16^a,b	2.44±0.16^a,b	2.86±0.23^a	2.12±0.13^b
T-bili (mg/dl)	0.3–0.9	0.29±0.02	1	0.77±0.04	0.81±0.04	0.70±0.04	0.77±0.05	0.80±0.04
			3	0.79±0.09	0.66±0.06	0.94±0.09	0.80±0.08	0.76±0.08
			6	0.30±0.02^b	0.32±0.02^a,b	0.29±0.01^b	0.36±0.02^a	0.29±0.01^b
Mean body weight (g)	–	37.57±1.02		40.34±0.98	37.16±1.70	36.83±0.97	37.83±1.03	40.28±0.68
Liver weight (g)	–	2.11±0.07		2.42±0.11	2.23±0.12	2.19±0.11	2.23±0.08	2.29±0.11
Relative liver weight	–	5.62±0.08		5.99±0.27	6.01±0.35	5.97±0.24	5.92±0.18	5.67±0.31

Data are presented as mean ± standard deviation (n=8).

^a,b,c

Represent comparisons within the same row, where different superscripts indicate significant differences at P<0.05. Referenced ranges of all serum biochemical components listed refer to Clinical chemistry of the laboratory mouse in The Mouse in Biomedical Research. The treatment groups were mice injected with CCl₄ twice weekly and subjected to daily gavage-feeding of H₂O (vehicle control), silymarin (200 mg/kg BW), a low (20 mg/kg BW), middle (100 mg/kg BW) or high dose (200 mg/kg BW) of LPE. The normal control group mice were not injected with CCl₄. Serum samples were collected and analyzed at 1, 3 and 6 weeks. Body and liver weight were measured on the date of euthanasia and relative liver weight was calculated as the proportion of liver weight to body weight. LPE, litchi pericarp extract; CCl₄, carbon tetrachloride; BW, body weight; GOT, glutamic oxaloacetate transaminase; GPT, glutamic pyruvic transaminase; TG, triglycerides; T-chol, total cholesterol; T-p, total protein; T-bili, total bilirubin.