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. 2017 Apr 26;13(6):3309–3314. doi: 10.3892/etm.2017.4387

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Copyright: © Lu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — miR-186 inhibits prostate cancer cell proliferation. (A) The miR-186 expression level in PC-3 cells after transfection with pCDH-miR-186 or pCDH were detected using quantitative polymerase chain reaction, where pCDH acted as a negative control. (B) The cell cycle phases of PC-3 cell transfection with pCDH-miR-186 or pCDH were analyzed using flow cytometry, where pCDH acted as a negative control. (C) Effect on cell proliferation of miR-186 overexpression in PC-3 cells was determined by the Cell Counting Kit-8 assay, where pCDH acted as a negative control. *P<0.05, **P<0.01 and ***P<0.001. miRNA, microRNA.