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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Methods Enzymol. 2016 Nov 11;587:97–113. doi: 10.1016/bs.mie.2016.09.055

Fig. 2.

Fig. 2

Graphical representation of plasmids used in this chapter. (A) pGEX-4T-1-LC3B for LC3B (1–120) expression in E. coli. (B) pFastBac-GST-ATG7 to construct bacmid for ATG7 expression in insect cells. (C) pGEX-TEV-ATG3 for ATG3 expression in E. coli. (D) pGEXTEV-ATG16L1 for ATG16L1 (1–69) expression in E. coli. (E) pLIB plasmid containing a GSTTEV-ATG12 (52–140), His-TEV-ATG5, ATG10, or ATG7 expression cassette to generate pBIG1a plasmid. (F) pBIG1a-ATG12-ATG5-ATG10-ATG7 plasmid to construct bacmid for ATG12 (52–140)~ATG5 expression in insect cells.