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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Methods Enzymol. 2016 Nov 11;587:97–113. doi: 10.1016/bs.mie.2016.09.055

Fig. 6.

Fig. 6

15% SDS-PAGE showing ATG12 (52–140)~ATG5-ATG16L1 (1–69) complex at different stages of purification. Lane M shows the molecular weight markers. Lane a shows elution from nickel-affinity chromatography, capturing His- and His-MBP tags on ATG5 and ATG16L1 (1–69), respectively. This is the second step in the purification, following an initial purification by glutathione affinity chromatography to capture GST-tag on ATG12. Lane b shows product of TEV cleavage reaction preformed during dialysis. Lane c shows final purified ATG12 (52–140)~ATG5-ATG16L1 (1–69) complex after subsequent cation ion exchange and gel filtration chromatography.