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. 2017 Apr 29;9(5):153. doi: 10.3390/toxins9050153

Figure 2.

Figure 2

The composition of the growth medium has an impact on EibG protein expression in STEC and on other Eib proteins in E. coli. EibG-positive STEC, as well as ECOR2 and ECOR9, were cultivated with agitation at 180 rpm and under static growth conditions (0 rpm) at 37 °C in the following media: LB broth as a complex medium, yeast extract medium (HM) and minimal medium (M9). For the distinctive differentiation of EibG expression levels, various protein amounts (in the graph interrupted by a line) of 2 µg and 7 µg for strain 2875/96 (α-type), 4 µg and 7 µg for strain 0520/99 (γ-type), and 1 µg and 7 µg for strain 3671/97 (harbouring genes eibC and eibG) were separated and immunoblotted, as indicated earlier. For ECOR2 and ECOR9 strains, 3 µg and 5 µg of proteins were loaded, respectively. Sizes of the marker proteins are indicated (M).