Figure 3.

Overview of the NMR data, defining the secondary structure of Ueq 12-1. Hydrogen-deuterium exchange rates of HN protons with solvent (H-D ex), ³J_HNHα couplings, secondary structure of Ueq 12-1 (SS) and NOE connectivities (d_ij) are shown versus the protein sequence. Widths of the bars correspond to the relative intensity of cross-peak in the 80 ms NOESY spectrum. Circles denote H_N protons, which exchange with solvent with characteristic times longer than 15 min. Black, gray and white rectangles denote residues with large (>8 Hz), medium (6–8 Hz) or small (<6 Hz) magnitudes of ³J_HNHα, respectively. Elements of secondary structure are shown on the separate line, β-strands are indicated by arrows, color of arrows indicates the involvement of strand in parallel (grey) or antiparallel (white) β-sheet.