. 2017 May 18;9(5):169. doi: 10.3390/toxins9050169

Table 2.

Solvent mixtures tested for extraction step optimization, recovery factors (%) and RSD of repeatability (%). Results for aflatoxin B1 (AFB1) and OTA were grouped, as well as for FB1, DON, T-2, and HT-2 toxins and ZEA.

Extraction Solvent	Recovery Factors (RSD_r ^a)
Extraction Solvent	AFB1, OTA	FB1, DON, T-2 and HT-2, ZEA
CH₃OH:H₂O 80:20	75 (20)	30 (19)
CH₃CN:H₂O:CH₃COOH 79:20:1	65 (15)	80 (15)
H₂O 4mL + EtOH 8mL	25 (20)	80 (19)

^a Relative standard deviation, calculated on six replicates processed under repeatability operating conditions.