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. 2017 May 19;9(5):171. doi: 10.3390/toxins9050171

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Secretory Phospholipase A₂ was measured by its ability to cleave a fluorogenic peptide substrate EnzChek^® (Cat# E10217). Column graph of sPLA₂ activity assay of D. typus and T. mossambicanus obtained from normalisation of slope values. There is a highly significant difference in activity between D. typus from the positive control and between D. typus and T. mossambicanus (p ≤ 0.001). There is also a significant difference of T. mossambicanus from the positive control (p ≤ 0.01). X axis: species name and concentration; Y axis: slope as a relative percentage. Analysis of triplicates was conducted on GraphPad PRISM 7.0 and error bars indicate standard deviation.