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Binding 3-mAb:FP and 4-mAb:FP immune complexes to RBCs in vitro. Washed Swiss Webster RBCs were incubated with RI-BoNT/A bound to 3-mAb:FP (left) or 4-mAb:FP (right). Complex binding to RBCs was detected using an APC-conjugated anti-human IgG and flow cytometry. Tracings include RBCs only (red), RBCs with the APC anti-human IgG (blue), and RBCs with the BoNT/A complexes and the APC anti-human IgG (orange).
