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IBET treatment increases p27 protein stability. (A) BON cells were treated with DMSO or IBET for 3 days, followed by addition of 20 μg/ml cycloheximide in the respective medium, and the cells were collected at 0, 2, 4 and 6 hr after the cycloheximide treatment of Western blotting with the indicated antibodies. (B) p27 protein band in (A) was scanned, and was quantitated using Image J and plotted as % protein relative to the signal value at 0 min.
