Figure 5.

(A) Western blotting was performed to examine levels of EZH2 protein in osteosarcoma cell lines compared with the normal human osteoblast cell line hFOB. **P<0.01 vs. hFOB. (B) WT or MT of EZH2 3′UTR was then cloned into the downstream of the firefly luciferase coding region of pMIR-GLOTM Luciferase vector. (C) U2OS cells were co-transfected with WT- or MT-EZH2-3′UTR luciferase reporter plasmid, and miR-NC or miR-137 mimic, respectively. The luciferase activity was examined. **P<0.01 vs. miR-NC. miR, microRNA; EZH2, enhancer of zeste homologue 2; UTR, untranslated region; NC, negative control; WT, wild-type; MT, mutant type; hFOB, human foetal osteoblastic cells.