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. 2017 Apr 20;13(6):2685–2690. doi: 10.3892/etm.2017.4365

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Direct neurogenic reprogramming. (A) Induced neurocytes exhibited typical neuron morphology with soma, dendrites and axons (magnification, ×100). (B) Nestin and MAP2 markers were identified by immunofluorescence staining (magnification ×100). (C) Reverse transcription-quantitative polymerase chain reaction analysis of MAP2 and nestin expression levels in differentiated iPS cells. (D) Procedure of direct neurogenic reprogramming. Data are expressed as mean ± standard deviation (n=3 in each group). *P<0.05 vs. fibroblast control group. MAP2, microtubule-associated protein 2; iPS, induced pluripotent stem; Sox2, sex determining region Y-box 2; NEUROD1, neurogenic differentiation 1; HVJ-E, hemagglutinating virus of Japan envelope; DMEM, Dulbecco's modified Eagle medium; FBS, fetal bovine serum.