Figure 4.

Effect of HDW and/or ATO on the activation of the DR4 and DR5 signaling pathway. (A) WEHI-3 cells treated with HDW at 0.4, 0.8 and 1.6 mg/ml for 48 h. (B) WEHI-3 cells were treated with 1.6 mg/ml HDW and 1.6 µM ATO or a combination of HDW and ATO for 48 h. The protein expression of DR4, DR5 and β-actin was measured by western blotting. Western blotting data presented are representative of those obtained in at least three separate experiments. HDW, Hedyotis diffusa Willd; ATO, arsenic trioxide; DR, death receptor.