Figure 6.

Effect of HDW and/or ATO on apoptosis-related proteins. WEHI-3 cells were treated with 1.6 mg/ml HDW, 1.6 µM ATO or a combination of HDW and ATO for 48 h. (A) The protein expression of PARP, and caspase-3, −8, and −9 was measured by western blotting. (B) The protein expression of Bcl-2, Bcl-xL and survivin was also measured by western blotting, as well as the protein expression of (C) Bak and Bid. Western blot data presented are representative of those obtained in at least three separate experiments. HDW, Hedyotis diffusa Willd; ATO, arsenic trioxide; PARP, poly adenosine diphosphate ribose polymerase; Bcl, B-cell lymphoma; xL, extra large.