Table I.
Gene-specific primer pairs.
| Gene | Primer sequence (5′ to 3′) | Amplicon (bp) |
|---|---|---|
| Beclin-1 | F: CAGTGTTGTTGCTCCATGCT | 202 |
| R: TGCACACAGTCCAGAAAAGC | ||
| LC3 | F: TTCTTCCTCCTGGTGAATGG | 253 |
| R: GTGGGTGCCTACGTTCTGAT | ||
| GAPDH | F: TGGAGTCTACTGGCGTCTT | 138 |
| R: TGTCATATTTCTCGTGGTTCA | ||
| p53 | F: CCTCACCATCATCACACTGGAAGAC | 91 |
| R: GTCTCTCCCAGGACAGGCACAAAC | ||
| AMPK | F: ACAGGAGAATAATGAATGAAGCCAA | 203 |
| R: ATGCCATTTTGCTTTCCTTACACCT | ||
| TSC1 | F: TTGACTGTTGTAATGACGGGTGCTC | 131 |
| R: TCCACCACCTCTGCTTCCACTACT | ||
| TSC2 | F: ACGAGTCAAACAAGCCAATCCTG | 97 |
| R: TTGTGGGTGTCGTATGATGGGAT | ||
| mTOR | F: AAAACCTCAGCATCCAGAGATACGC | 191 |
| R: CATCAGAGTCAAGTGGTCATAGTCCG | ||
| sestrin2 | F: TACAATACCATCGCCATGCACAGT | 121 |
| R: GGTTCACCTCCCCATAATCATAGTCA | ||
| β-actin | F: ACTTAGTTGCGTTACACCCTT | 156 |
| R: GTCACCTTCACCGTTCCA |
F, forward; R, reverse; LC, light chain; p53, tumor suppressor p53; AMPK, adenosine monophosphate-activated protein kinase; TSC, tuberous sclerosis protein; mtor, mechanistic target of rapamycin.