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. 2017 May 15;144(10):1807–1819. doi: 10.1242/dev.141911

Fig. 3.

Fig. 3.

Gross morphological development of GABA motor neurons and muscles does not require cholinergic innervation. (A-C′) Confocal images of the ventral nerve cord and body wall muscles posterior to the vulva of an adult wild-type (A,A′), unc-3(e151) mutant (B,B′) and acr-2(L/S) transgenic (C,C′) animals expressing a membrane-bound GFP in the distal row of body wall muscles (him4p::mCD8::GFP) (A-C) or mCherry in the GABA nervous system (unc-47p::mCherry) (A′-C′). Arrows indicate muscle arm shafts and arrowheads indicate muscle arm termini. Asterisks indicate GABA neuron commissures. Scale bars: 10 µm (A-C); 20 µm (A′-C′). (D) Quantification of the percentage of the ventral nerve cord region covered by muscle membrane. ns, not significant; ANOVA with Dunnett's multiple comparisons test.