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. 2017 May 15;144(10):1807–1819. doi: 10.1242/dev.141911

Fig. 5.

Fig. 5.

Vesicular release from cholinergic motor neurons shapes GABA synaptic outputs. (A) Confocal images showing the dorsal nerve cord of eight animals co-expressing ACR-12::GFP in GABA neurons and tetanus toxin in cholinergic neurons. Scale bar: 5 µm. (B) Quantification of the average number of receptor clusters per 85 µm of dorsal nerve cord for the genotypes indicated, normalized to wild type. (C,D) Merged confocal images of unc-47p::mCherry::RAB-3 and UNC-49::GFP labeling in the ventral nerve cord of adult wild-type (C) and transgenic animals with specific TetTx expression in cholinergic neurons (D). Brackets indicate areas devoid of synaptic clusters. Scale bars: 20 µm. (E) Average number of pre- and postsynaptic clusters per 50 µm for each genotype, normalized to wild type. (F) Average area of pre- and postsynaptic clusters at GABA synapses for each genotype as indicated. ***P<0.001, ****P<0.0001; Student's t-test.