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. 2017 May 15;144(10):1831–1840. doi: 10.1242/dev.146936

Fig. 2.

Fig. 2.

HFR1 promotes PIF1 degradation in the dark. (A) Immunoblot shows higher abundance of PIF1 in the hfr1 and cop1-4 hfr1 backgrounds compared with wild-type seedlings. Four-day-old dark-grown seedlings were used for protein extraction. The blot was probed with anti-PIF1 and anti-RPT5 antibodies. (B) Quantification of PIF1 protein level using RPT5 as a control. The letters a-d indicate statistically significant differences between means of protein levels (P<0.05) based on two-way ANOVA analyses. Error bars indicate s.d. (n=7). (C) Immunoblots show the PIF1 (top panel) and GFP-HFR1 (middle panel) and loading control RPT5 (bottom panel) levels in wild-type Col-0, cop1-4, cop1-4 hfr1, cop1-4 hfr1/GFP-HFR1 and cop1-4 hfr1/GFP-HFR1*. Immunoblot was performed as described in A. (D) Quantification of PIF1 protein level using RPT5 as a control. The letters a-d indicate statistically significant differences between means of protein levels (P<0.05) based on two-way ANOVA analyses. Error bars indicate s.d. (n=3).